h14 antibody Search Results


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Figure 2 HIV proteins expression in HIV Tg mice. (A) <t>gp120</t> expression in HIV Tg mice. Protein lysates from mice spleen and lymph node were separated by SDS-PAGE and analyzed by western blot using anti-gp120 antibody as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT is the mouse control FVB/N, Tg is an HIV Tg mice without skin lesions, mice number 18, 21, and 22 represent mice at the pre-lymphadenopathy stage while mice number 11, 20 and 35 represent mice at a late stage of lymphadenopathy. The numbers in the right represent the protein molecular weight in KDa. (B) Histogram showing the fold changes in gp120 expression in the lymph node versus the spleen from the same mouse. The relative intensity of gp120 was quantified by densitometry and normalized with the beta actin. (C) Western blot for p17 and nef proteins expression in HIV Tg mice using anti-p17 antibody and HIV-1 Nef antiserum as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT are mice wild type control FVB/N, Tg are HIV Tg mice without skin lesions, Tg pre-L are mice at pre-lymphadenopathy stage and Tg-L are mice at the last stage of lymphadenopathy. The protein molecular weights are represented in the right of the figure.
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Figure 2 HIV proteins expression in HIV Tg mice. (A) <t>gp120</t> expression in HIV Tg mice. Protein lysates from mice spleen and lymph node were separated by SDS-PAGE and analyzed by western blot using anti-gp120 antibody as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT is the mouse control FVB/N, Tg is an HIV Tg mice without skin lesions, mice number 18, 21, and 22 represent mice at the pre-lymphadenopathy stage while mice number 11, 20 and 35 represent mice at a late stage of lymphadenopathy. The numbers in the right represent the protein molecular weight in KDa. (B) Histogram showing the fold changes in gp120 expression in the lymph node versus the spleen from the same mouse. The relative intensity of gp120 was quantified by densitometry and normalized with the beta actin. (C) Western blot for p17 and nef proteins expression in HIV Tg mice using anti-p17 antibody and HIV-1 Nef antiserum as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT are mice wild type control FVB/N, Tg are HIV Tg mice without skin lesions, Tg pre-L are mice at pre-lymphadenopathy stage and Tg-L are mice at the last stage of lymphadenopathy. The protein molecular weights are represented in the right of the figure.
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Biozol Diagnostica Vertrieb GmbH mutation-specific antibody
Figure 2 HIV proteins expression in HIV Tg mice. (A) <t>gp120</t> expression in HIV Tg mice. Protein lysates from mice spleen and lymph node were separated by SDS-PAGE and analyzed by western blot using anti-gp120 antibody as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT is the mouse control FVB/N, Tg is an HIV Tg mice without skin lesions, mice number 18, 21, and 22 represent mice at the pre-lymphadenopathy stage while mice number 11, 20 and 35 represent mice at a late stage of lymphadenopathy. The numbers in the right represent the protein molecular weight in KDa. (B) Histogram showing the fold changes in gp120 expression in the lymph node versus the spleen from the same mouse. The relative intensity of gp120 was quantified by densitometry and normalized with the beta actin. (C) Western blot for p17 and nef proteins expression in HIV Tg mice using anti-p17 antibody and HIV-1 Nef antiserum as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT are mice wild type control FVB/N, Tg are HIV Tg mice without skin lesions, Tg pre-L are mice at pre-lymphadenopathy stage and Tg-L are mice at the last stage of lymphadenopathy. The protein molecular weights are represented in the right of the figure.
Mutation Specific Antibody, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Babco Inc phosphorylated ctd at ser5 (h14)
Figure 2 HIV proteins expression in HIV Tg mice. (A) <t>gp120</t> expression in HIV Tg mice. Protein lysates from mice spleen and lymph node were separated by SDS-PAGE and analyzed by western blot using anti-gp120 antibody as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT is the mouse control FVB/N, Tg is an HIV Tg mice without skin lesions, mice number 18, 21, and 22 represent mice at the pre-lymphadenopathy stage while mice number 11, 20 and 35 represent mice at a late stage of lymphadenopathy. The numbers in the right represent the protein molecular weight in KDa. (B) Histogram showing the fold changes in gp120 expression in the lymph node versus the spleen from the same mouse. The relative intensity of gp120 was quantified by densitometry and normalized with the beta actin. (C) Western blot for p17 and nef proteins expression in HIV Tg mice using anti-p17 antibody and HIV-1 Nef antiserum as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT are mice wild type control FVB/N, Tg are HIV Tg mice without skin lesions, Tg pre-L are mice at pre-lymphadenopathy stage and Tg-L are mice at the last stage of lymphadenopathy. The protein molecular weights are represented in the right of the figure.
Phosphorylated Ctd At Ser5 (H14), supplied by Babco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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HiSS Diagnostics h5/h14 antibody
Figure 2 HIV proteins expression in HIV Tg mice. (A) <t>gp120</t> expression in HIV Tg mice. Protein lysates from mice spleen and lymph node were separated by SDS-PAGE and analyzed by western blot using anti-gp120 antibody as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT is the mouse control FVB/N, Tg is an HIV Tg mice without skin lesions, mice number 18, 21, and 22 represent mice at the pre-lymphadenopathy stage while mice number 11, 20 and 35 represent mice at a late stage of lymphadenopathy. The numbers in the right represent the protein molecular weight in KDa. (B) Histogram showing the fold changes in gp120 expression in the lymph node versus the spleen from the same mouse. The relative intensity of gp120 was quantified by densitometry and normalized with the beta actin. (C) Western blot for p17 and nef proteins expression in HIV Tg mice using anti-p17 antibody and HIV-1 Nef antiserum as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT are mice wild type control FVB/N, Tg are HIV Tg mice without skin lesions, Tg pre-L are mice at pre-lymphadenopathy stage and Tg-L are mice at the last stage of lymphadenopathy. The protein molecular weights are represented in the right of the figure.
H5/H14 Antibody, supplied by HiSS Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Babco Inc monoclonal antibodies h14
Figure 2 HIV proteins expression in HIV Tg mice. (A) <t>gp120</t> expression in HIV Tg mice. Protein lysates from mice spleen and lymph node were separated by SDS-PAGE and analyzed by western blot using anti-gp120 antibody as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT is the mouse control FVB/N, Tg is an HIV Tg mice without skin lesions, mice number 18, 21, and 22 represent mice at the pre-lymphadenopathy stage while mice number 11, 20 and 35 represent mice at a late stage of lymphadenopathy. The numbers in the right represent the protein molecular weight in KDa. (B) Histogram showing the fold changes in gp120 expression in the lymph node versus the spleen from the same mouse. The relative intensity of gp120 was quantified by densitometry and normalized with the beta actin. (C) Western blot for p17 and nef proteins expression in HIV Tg mice using anti-p17 antibody and HIV-1 Nef antiserum as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT are mice wild type control FVB/N, Tg are HIV Tg mice without skin lesions, Tg pre-L are mice at pre-lymphadenopathy stage and Tg-L are mice at the last stage of lymphadenopathy. The protein molecular weights are represented in the right of the figure.
Monoclonal Antibodies H14, supplied by Babco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Babco Inc mouse monoclonal antibody h14
Figure 2 HIV proteins expression in HIV Tg mice. (A) <t>gp120</t> expression in HIV Tg mice. Protein lysates from mice spleen and lymph node were separated by SDS-PAGE and analyzed by western blot using anti-gp120 antibody as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT is the mouse control FVB/N, Tg is an HIV Tg mice without skin lesions, mice number 18, 21, and 22 represent mice at the pre-lymphadenopathy stage while mice number 11, 20 and 35 represent mice at a late stage of lymphadenopathy. The numbers in the right represent the protein molecular weight in KDa. (B) Histogram showing the fold changes in gp120 expression in the lymph node versus the spleen from the same mouse. The relative intensity of gp120 was quantified by densitometry and normalized with the beta actin. (C) Western blot for p17 and nef proteins expression in HIV Tg mice using anti-p17 antibody and HIV-1 Nef antiserum as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT are mice wild type control FVB/N, Tg are HIV Tg mice without skin lesions, Tg pre-L are mice at pre-lymphadenopathy stage and Tg-L are mice at the last stage of lymphadenopathy. The protein molecular weights are represented in the right of the figure.
Mouse Monoclonal Antibody H14, supplied by Babco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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N/A
Reacts with the H14 protein of influenza A virus. Cross-reactivity to other subtypes not tested.Store at 4 oC; DO NOT FREEZE; Stable for 6 months from the date of shipment. Non-hazardous.http://www.creative-diagnostics.com/Anti-H14-Protein-Antibody-54132-147.htm
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NEURON SPECIFIC ENOLASEin frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before
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Figure 2 HIV proteins expression in HIV Tg mice. (A) gp120 expression in HIV Tg mice. Protein lysates from mice spleen and lymph node were separated by SDS-PAGE and analyzed by western blot using anti-gp120 antibody as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT is the mouse control FVB/N, Tg is an HIV Tg mice without skin lesions, mice number 18, 21, and 22 represent mice at the pre-lymphadenopathy stage while mice number 11, 20 and 35 represent mice at a late stage of lymphadenopathy. The numbers in the right represent the protein molecular weight in KDa. (B) Histogram showing the fold changes in gp120 expression in the lymph node versus the spleen from the same mouse. The relative intensity of gp120 was quantified by densitometry and normalized with the beta actin. (C) Western blot for p17 and nef proteins expression in HIV Tg mice using anti-p17 antibody and HIV-1 Nef antiserum as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT are mice wild type control FVB/N, Tg are HIV Tg mice without skin lesions, Tg pre-L are mice at pre-lymphadenopathy stage and Tg-L are mice at the last stage of lymphadenopathy. The protein molecular weights are represented in the right of the figure.

Journal: Retrovirology

Article Title: B cell lymphoma in HIV transgenic mice.

doi: 10.1186/1742-4690-10-92

Figure Lengend Snippet: Figure 2 HIV proteins expression in HIV Tg mice. (A) gp120 expression in HIV Tg mice. Protein lysates from mice spleen and lymph node were separated by SDS-PAGE and analyzed by western blot using anti-gp120 antibody as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT is the mouse control FVB/N, Tg is an HIV Tg mice without skin lesions, mice number 18, 21, and 22 represent mice at the pre-lymphadenopathy stage while mice number 11, 20 and 35 represent mice at a late stage of lymphadenopathy. The numbers in the right represent the protein molecular weight in KDa. (B) Histogram showing the fold changes in gp120 expression in the lymph node versus the spleen from the same mouse. The relative intensity of gp120 was quantified by densitometry and normalized with the beta actin. (C) Western blot for p17 and nef proteins expression in HIV Tg mice using anti-p17 antibody and HIV-1 Nef antiserum as described under “Experimental Procedures”. The mice analyzed are indicated on the top of the figure: WT are mice wild type control FVB/N, Tg are HIV Tg mice without skin lesions, Tg pre-L are mice at pre-lymphadenopathy stage and Tg-L are mice at the last stage of lymphadenopathy. The protein molecular weights are represented in the right of the figure.

Article Snippet: Proteins (30 μg) were electrophoresed (12% SDS-polyacrylamide), transferred to a PVDF membrane (Bio-Rad), probed with anti-HIV-1 gp120 (Aalto Bio Reagents Ltd., Ireland), anti-HIV-1 p17 (Abnova), HIV-1 Nef Antiserum (NIH AIDS Research and Reference Reagent Program), anti-beta actin (Cell Signaling) and antimouse serum albumin (Abcam), incubated with respective HRP-conjugated secondary antibodies (Santa Cruz, Inc., CA), developed using an ECL chemiluminescent substrate kit (Invitrogen) and exposed to Kodak X-ray film.

Techniques: Expressing, SDS Page, Western Blot, Control, Molecular Weight